Abstract
In many organisms, the rate of the genetic recombination of Danio rerio is not uniform along chromosomes or between sexes. To compare the relative rates of recombination during meiosis in male and female zebrafish, we constructed a genetic map based on male meiosis. We developed a meiotic mapping panel of 94 androgenetic haploid embryos that were scored for genetic polymorphisms. The resulting male map was compared to the average female and sex maps.
We found that the rate of recombination in male meiosis is dramatically suppressed relative to that in female meiosis, especially near the centromere. These findings have practical applications for experimental design. The use of female-only meiosis in a positional cloning project maximizes the ratio of genetic map distance to physical distance. Alternatively, the use of male-only meiosis to locate a mutation initially in a linkage group or to maintain linked allele relationships minimizes recombination, facilitating some types of analysis.
Product Information
Purity: > 90% as determined by SDS-PAGE
Endotoxin: < 1.0 EU per μg of protein as determined by the LAL method
Activity:
- Measured in a cell proliferation assay using human umbilical vein endothelial cells (HUVEC).
- The ED50 for this effect is typically 30 to 250 ng/mL.
Protein Construction: A DNA sequence encoding zebrafish VEGF165 (O73682-Isoform VEGF165) (Met 1-Arg188) was expressed and purified.
Accession number: O73682
Expressed Host: Baculovirus-insect cells
Species: Danio rerio (zebrafish)
Planned N Terminal: Wing 24
Molecular mass: Recombinant zebrafish VEGF165 consists of 165 amino acids and has a calculated molecular mass of 19.2 kDa. It migrates as a 22 KDa band on SDS-PAGE under reducing conditions.
Formulation
- Lyophilized from sterile 20 mM Tris, 500 mM NaCl, pH 7.0
- Please contact us with any concerns or special requirements.
- Typically 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants prior to lyophilization.
Shipping
Recombinant proteins are generally provided as a lyophilized powder that is shipped at room temperature. Recombinant protein bulk packs are provided as a frozen liquid. They are shipped with blue ice unless customers require otherwise.
Stability and Storage
Samples are stable for up to twelve months from the date of receipt at -20℃ to -80℃. Store it under sterile conditions between -20℃ and -80℃. It is recommended to divide the protein into aliquots for optimal storage. Avoid repeated cycles of freezing and thawing.
Reconstitution
A printed copy of the COA with instructions for reconstitution is shipped with the products. Please refer to it for detailed information.
